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Microsomes were prepared by sonication of 10 8 cells, removal of debris by centrifugation at 10,000 g for 15 min, and subsequent centrifugation of the resulting supernatant at 100,000 g for 60 min

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Research shows that orexin, a neurochemical produced by the brain, plays an important role in the regulation of the sleep cycle

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169 Heparin-lidocaine combined significantly reduced the percentage of bladder pain (38% versus 13%